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基本信息
載體類(lèi)型: | 酵母雙雜交載體 |
載體抗性: | Kan |
篩選標(biāo)記: | TRP1 |
啟動(dòng)子: | ADH1 |
載體大小: | 8.3kb |
5' 測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3' 測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
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質(zhì)粒圖譜和多克隆位點(diǎn)信息
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載體描述
Yeast two-hybrid systems provide a sensitive method for detecting relatively weak and transient protein interactions. Such interactions may not be biochemically detectable, but may be critical for proper functioning of complex biological systems. pGBKT7-53 and pGADT7-T encode fusions between the GAL4 DNA-BD and AD and murine p53 and SV40 large T-antigen, respectively. p53 and large T-antigen interact in a yeast two-hybrid assay.