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基本信息
載體類型: | 酵母單雜交載體 |
載體抗性: | Kan |
篩選標(biāo)記: | TRP1 |
啟動(dòng)子: | ADH1 |
載體大小: | 7.9kb |
5' 測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3' 測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
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質(zhì)粒圖譜和多克隆位點(diǎn)信息
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載體描述
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.